Beijing Hotgen Biotech Co., Ltd.
Beijing Hotgen Biotech Co., Ltd.

Enzyme-linked Immunosorbent Assay (ELISA) Kit

Hotgen’s ELISA products cover many diseases, such as rheumatoid arthritis (Anti-CCP), liver cancer (AFP and DCP), and bone metabolism disease (25-OH-VD). These products well tested across time are providing reliable diagnostic results of critical clinical values to medical institutions at economical rates.
Operation Hotgen’s Procedures of Enzyme-linked Immunosorbent Assay (ELISA) Products

Operation Hotgen’s Procedures of Enzyme-linked Immunosorbent Assay (ELISA) Products

  • Sample Preparation: Collect and process the samples (e.g. serum, plasma)

  • Sample Addition: Add samples and standards to the wells of the microplate

  • Incubation: Incubate the microplate at an appropriate temperature

  • Washing: Wash the plate with wash buffer

  • Add Enzyme Conjugate: Add enzyme-labeled secondary antibody or enzyme conjugate to the microplate

  • Second Incubation and Washing: Repeat incubation and washing steps to ensure sufficient reaction of the conjugate

  • Substrate Addition: Add the substrate solution to the microplate

  • Stop Reaction: Add the stop solution to halt the enzyme reaction

  • Results Reading: Measure the absorbance using a microplate reader


What's the Benefits of Using Hotgen’s Procedures of Enzyme-linked Immunosorbent Assay (ELISA) Products?

What's the Benefits of Using Hotgen’s Procedures of Enzyme-linked Immunosorbent Assay (ELISA) Products?

  • Economy: Competitively priced, reducing financial burden without compromising quality.

  • Reliability: Ensures accurate and reliable diagnostic results.

  • Broad Application: Available to diagnose various conditions, including rheumatoid arthritis, liver diseases, sex hormones, cardiovascular diseases, bone metabolism, and anemia.

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